Data on pharmacological applications and hypothermia protection against in vitro oxygen-glucose-deprivation-related neurodegeneration of adult rat CA1 region
نویسندگان
چکیده
In this data article, the level of chemical neuroprotection against oxygen-glucose-deprivation (OGD)-related neurodegeneration in CA1 was analyzed using the measurements on CA1 stratum pyramidale (CA1sp) width. Adult rat hippocampal slices were incubated in OGD medium for 60 min to create a model for severe ischemic conditions. Alternatively, control slices were incubated in artificial cerebrospinal fluid (ACSF) for 60 min. A study of OGD induced neurodegeneration and partial prevention by pharmacological agents reported; baclofen, memantine and l-carnitine effects were included. Also, the use of hypothermia was reported (P. Öz, H. Saybaşılı, 2016) [1]. Here, the use CA1sp width measurements on Nissl-stained hippocampal slices is introduced as a valid and affordable method for detecting the level of neurodegeneration and neuroprotection on hippocampal slices. The protective effect of hypothermia was found to be more pronounced compared to other agents.
منابع مشابه
Protective effect of Viola tricolor and Viola odorata extracts on serum/glucose deprivation-induced neurotoxicity: role of reactive oxygen species
Objective: Oxidative stress plays a key role in the pathophysiology of brain ischemia and neurodegenerative disorders.Previous studies indicated that Viola tricolor and Viola odorataare rich sources of antioxidants. This study aimed to determine whether these plants protect neurons against serum/glucose deprivation (SGD)-induced cell death in an in vitro model of ischemia and neurodegeneration....
متن کاملGlutamate and non-glutamate receptor mediated toxicity caused by oxygen and glucose deprivation in organotypic hippocampal cultures.
In vitro ischemia models have utilized oxygen, or oxygen and glucose deprivation to simulate ischemic neuronal injury. Combined oxygen and glucose deprivation can induce neuronal damage which is in part mediated through NMDA receptors. Severe oxygen deprivation alone however can cause neuronal injury which is not NMDA mediated. We tested the hypothesis that NMDA, or non-NMDA receptor mediated m...
متن کاملNimodipine Protects PC12 Cells against Oxygen-Glucose Deprivation
The protective effect of a L-type calcium channel blocker, nimodipine, on cell injury induced by oxygen-glucose deprivation (OGD) in PC12 cells was investigated. PC12 cells were exposed to in-vitro oxygen-glucose deprivation (30 minutes and 60 minutes respectively) in the presence or absence of nimodipine (10mM/L) in three different time schedules (pre-24h, pre-3h and concurrently). Cellular vi...
متن کاملP2 receptor antagonist trinitrophenyl-adenosine-triphosphate protects hippocampus from oxygen and glucose deprivation cell death.
In this work, we mainly used the organotypic model of rat hippocampus to demonstrate the protective role of the P2 receptor antagonist trinitrophenyl-adenosine-triphosphate (TNP-ATP) during oxygen/glucose deprivation. Among the P2X receptors that TNP-ATP specifically blocks, mainly P2X1 seems to be involved in the processes of cell damage after oxygen/glucose deprivation. P2X1 receptor is stron...
متن کاملHypothermia protects human neurons
BACKGROUND AND AIMS Hypothermia provides neuroprotection after cardiac arrest, hypoxic-ischemic encephalopathy, and in animal models of ischemic stroke. However, as drug development for stroke has been beset by translational failure, we sought additional evidence that hypothermia protects human neurons against ischemic injury. METHODS Human embryonic stem cells were cultured and differentiate...
متن کامل